IFN-γ: A therapeutic target for cancer therapy

Interferon gamma (IFN-γ) is a cytokine that induces a series of immune responses against viruses and tumors. The pro-apoptotic, anti-tumor and anti-proliferative actions of the cells are activated when IFN-γ binds to the IFN-γ receptor, which is made up of IFNGR1 and IFNGR2.

IFN-γ expression that is erroneous encourages the growth of tumors. IFN-γ has thus become recognized as a major contributor to the immunogenicity of malignancy.

The CD4+ T helper cells and CD8+ cytotoxic T cells are the effector T cells that release IFN-γ. Natural killer cells, natural killer T cells, and B cells are additional immune cells that secrete IFN-γ.

IFN-γ is stimulated by interleukins such IL-12, IL-15, IL-18, and IL-21. These interleukins’ stimulatory effects also result from intracellular signaling of transcription factors such as AP-1, JAK/STAT, and T-bet.

In PD-1/PD-L1 immune checkpoint inhibitor cancer treatment, IFN-γ is a prognostic marker. The production of IFN-γ is associated with an improved response to immune checkpoint blockade (ICB) therapy in triple-negative breast cancer, and is considered to be an emerging high-potential adjuvant therapy target.

Recombinant IFN-γ and Receptor Proteins

To facilitate the study of IFN- in cancer immunity, Sino Biological has produced a panel of recombinant IFN-γ protein and receptor reagents.

Featured IFN-γ and Receptor Proteins

Human IFN-γ Protein Cat#: 11725-HNAE

  • Tag-Free
  • HPLC-verified
  • High Activity
  • High-Purity

IFN-γ: A therapeutic target for cancer therapy

Purity: ≥ 95% as determined by SDS-PAGE & HPLC. Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Anti-viral assays using WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR1 Protein Cat#: 10338-H08H

IFN-γ: A therapeutic target for cancer therapy

Ability to inhibit rhIFN-γ mediated protection of WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Mouse IFN-γ Protein Cat#: 50709-MNAH

IFN-γ: A therapeutic target for cancer therapy

Immobilized Mouse Interferon Gamma can bind Mouse IFNGR1 (Cat: 50705-M02H). Image Credit: Sino Biological Inc.

IFN-γ: A therapeutic target for cancer therapy

Antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.

Human IFNGR2 Protein Cat#: 50665-M08H

IFN-γ: A therapeutic target for cancer therapy

Purity: > 97 % as determined by SDS-PAGE. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Image Credit: Sino Biological Inc.

Product list of Recombinant IFN-γ & Receptors

Table 1. Source: Sino Biological Inc.

Cat#MoleculeSpeciesExpressed HostPurityActivityTag
11725-HNAEIFNGHumanE. coli≥95 % (by SEC-HPLC)ActiveNative
11725-HNASHumanCHO Stable Cells>92%ActiveNative
50709-M02HMouseHEK293 Cells>92%ActiveC-hFc
50709-M08HMouseHEK293 Cells>75%ActiveC-His
50709-MNAHMouseHEK293 Cells>85%ActiveNative
60007-F08HFerretHEK293 Cells>95% C-His
80234-R02HRatHEK293 Cells>92%ActiveC-hFc
90008-C08HRhesusHEK293 Cells>90%ActiveC-His
90008-CNAERhesusE. coli>90%ActiveNative
10338-H02HIFNGR1HumanHEK293 Cells>95% C-hFc
10338-H08HHumanHEK293 Cells>97%ActiveC-His
50705-M02HMouseHEK293 Cells>95%ActiveC-hFc
50705-M08HMouseHEK293 Cells>98%ActiveC-His
80182-R02HRatHEK293 Cells>95%ActiveC-hFc
90207-C02HCynomolgusHEK293 Cells>95%ActiveC-hFc
90207-C08HCynomolgusHEK293 Cells>95% C-His
50665-M08HIFNGR2MouseHEK293 Cells>97% C-His

 

Other related proteins

  • PD-1
  • CD4
  • IL-12
  • PD-L1
  • IL-18
  • CD8
  • IL-15
  • IL-21

Antibodies for IFN-γ and Receptors

For use in ELISA, WB, FCM, and IHC, Sino Biological has created a panel of antibodies that target the IFN-γ cytokine and its receptors.

Anti-IFN-γ Antibody (FITC), Rabbit Mab Cat#: 11725-R003-F

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.

Anti-IFN-γ Antibody, Rabbit PAb Cat#: 105908-T08

IFN-γ: A therapeutic target for cancer therapy

Immunochemical staining of human IFNG in human kidney cell. Image Credit: Sino Biological Inc.

Anti-IFNGR1 Antibody (APC), Mouse MAb Cat#: 10338-MM05-A

IFN-γ: A therapeutic target for cancer therapy

Flow cytometric analysis of Human IFNGR1 expression on human whole blood lymphocytes. Image Credit: Sino Biological Inc.