Interferon gamma (IFN-γ) is a cytokine that induces a series of immune responses against viruses and tumors. The pro-apoptotic, anti-tumor and anti-proliferative actions of the cells are activated when IFN-γ binds to the IFN-γ receptor, which is made up of IFNGR1 and IFNGR2.
IFN-γ expression that is erroneous encourages the growth of tumors. IFN-γ has thus become recognized as a major contributor to the immunogenicity of malignancy.
The CD4+ T helper cells and CD8+ cytotoxic T cells are the effector T cells that release IFN-γ. Natural killer cells, natural killer T cells, and B cells are additional immune cells that secrete IFN-γ.
IFN-γ is stimulated by interleukins such IL-12, IL-15, IL-18, and IL-21. These interleukins’ stimulatory effects also result from intracellular signaling of transcription factors such as AP-1, JAK/STAT, and T-bet.
In PD-1/PD-L1 immune checkpoint inhibitor cancer treatment, IFN-γ is a prognostic marker. The production of IFN-γ is associated with an improved response to immune checkpoint blockade (ICB) therapy in triple-negative breast cancer, and is considered to be an emerging high-potential adjuvant therapy target.
To facilitate the study of IFN- in cancer immunity, Sino Biological has produced a panel of recombinant IFN-γ protein and receptor reagents.
Featured IFN-γ and Receptor Proteins
Human IFN-γ Protein Cat#: 11725-HNAE
- High Activity
Purity: ≥ 95% as determined by SDS-PAGE & HPLC. Image Credit: Sino Biological Inc.
Anti-viral assays using WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Human IFNGR1 Protein Cat#: 10338-H08H
Ability to inhibit rhIFN-γ mediated protection of WISH cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Mouse IFN-γ Protein Cat#: 50709-MNAH
Immobilized Mouse Interferon Gamma can bind Mouse IFNGR1 (Cat: 50705-M02H). Image Credit: Sino Biological Inc.
Antiviral assays using L929 cells infected with vesicular stomatitis virus (VSV). Image Credit: Sino Biological Inc.
Human IFNGR2 Protein Cat#: 50665-M08H
Purity: > 97 % as determined by SDS-PAGE. Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method. Image Credit: Sino Biological Inc.
Product list of Recombinant IFN-γ & Receptors
Table 1. Source: Sino Biological Inc.
|11725-HNAE||IFNG||Human||E. coli||≥95 % (by SEC-HPLC)||Active||Native|
|11725-HNAS||Human||CHO Stable Cells||>92%||Active||Native|
|60007-F08H||Ferret||HEK293 Cells||>95%|| ||C-His|
|10338-H02H||IFNGR1||Human||HEK293 Cells||>95%|| ||C-hFc|
|90207-C08H||Cynomolgus||HEK293 Cells||>95%|| ||C-His|
|50665-M08H||IFNGR2||Mouse||HEK293 Cells||>97%|| ||C-His|
Other related proteins
Antibodies for IFN-γ and Receptors
For use in ELISA, WB, FCM, and IHC, Sino Biological has created a panel of antibodies that target the IFN-γ cytokine and its receptors.
Anti-IFN-γ Antibody (FITC), Rabbit Mab Cat#: 11725-R003-F
Flow cytometric analysis of Human IFN-γ expression on human peripheral blood lymphocytes. Image Credit: Sino Biological Inc.
Anti-IFN-γ Antibody, Rabbit PAb Cat#: 105908-T08
Immunochemical staining of human IFNG in human kidney cell. Image Credit: Sino Biological Inc.
Anti-IFNGR1 Antibody (APC), Mouse MAb Cat#: 10338-MM05-A
Flow cytometric analysis of Human IFNGR1 expression on human whole blood lymphocytes. Image Credit: Sino Biological Inc.