High-quality cytokines for T-cell culture

Tumor-infiltrating lymphocytes and other T cell-mediated treatments, such as CAR-T, have the potential to improve the effectiveness and personalization of cancer care. Cytokines play a crucial part in T cell survival, development, and differentiation.

T cell therapies typically entail the removal of autologous T cells from patients, cell manipulation/engineering, culture expansion, and re-infusion into the patient. A single administration of adoptive T cell therapy can necessitate billions of expanded T cells.

To produce the highest output of T cells while maintaining the correct phenotypic and function, high-quality cytokines must be used.

A powerful cytokine called interleukin-2 (IL-2) is frequently utilized in T cell culture to encourage the proliferation and differentiation of T cells into effector and memory cells.

Other c-cytokines, including IL4, IL-7IL-15, and IL-21, play a vital role in memory T cell development, proliferation, and survival. Varying combinations of IL-2, IL-7, IL-15, and IL-21 have been shown to efficiently expand T cells, resulting in populations expressing early-differentiation markers, such as CD27, CD28, CD62L, and CD127. 

To enhance a particular T cell phenotype, culture conditions can be adjusted. For instance, Th1 needs IL-12, Th2 needs IL-4, Tregs need TGF-β1, and Th17 needs IL-6.

High-quality cytokines for T-cell culture

Image Credit: Sino Biological Inc.

To promote the use of cytokines in T cell-mediated therapies, Sino Biological has created an extensive collection of high-quality cytokines:

  • HPLC Verified
  • High Activity
  • Various Species
  • Low Endotoxin
  • High Purity

Featured Cytokines

Human IL-2 Protein Cat#: 11848-HNAH1-E

High-quality cytokines for T-cell culture

> 95 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Image Credit: Sino Biological Inc.

Human IL-4 Protein Cat#: 11846-HNAE

High-quality cytokines for T-cell culture

Cell proliferation assay using TF1 human erythroleukemic cells. Image Credit: Sino Biological Inc.

Human IL-7 Protein Cat#: 11821-HNAE

High-quality cytokines for T-cell culture

> 95 % as determined by SEC-HPLC. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Cell proliferation assay using anti-CD3 antibody activated human peripheral blood mononuclear cell (PBMC). Image Credit: Sino Biological Inc.

Human IL-21 Protein Cat#: 10584-HNAE

High-quality cytokines for T-cell culture

Ability to induce Interferon-gamma secretion by human natural killer lymphoma NK-92 cells. Image Credit: Sino Biological Inc.

Human IL-6 Protein Cat#: 10395-HNAE

High-quality cytokines for T-cell culture

Cell proliferation assay using TF-1 human erythroleukemic cells. Image Credit: Sino Biological Inc.

High-quality cytokines for T-cell culture

Immobilized Human IL-6 can bind Human IL-6R (Cat: 10398-H02H). Image Credit: Sino Biological Inc.

Human IL-15 Protein Cat#: 10360-HNCE

High-quality cytokines for T-cell culture

Cell proliferation assay using MO7e human megakaryocytic leukemic cells. Image Credit: Sino Biological Inc.

More Cytokines related to T Cell Culture

Source: Sino Biological Inc.

Cat#SpeciesCytokineExpression HostTagPurityActivity
11848-H08HHumanIL2HEK293 CellsC-His> 95 % 
11848-HNAH1-EHuman HEK293 CellsNative> 95 % (HPLC verified)Active
51061-MNAEMouse E. coliNative> 92 %Active
70014-DNAECanine E. coliNative> 95 %Active
80075-RNAERat E. coliNative> 95 %Active
11846-HNAEHumanIL4E. coliNative> 92 %Active
11846-HNAE-BHuman E. coliNative> 90 % 
51084-M08BMouse Baculovirus-Insect CellsC-His> 95 %Active
51084-MNAEMouse E. coliNative> 90 %Active
70021-DNAECanine E. coliNative> 96 %Active
10395-HNAEHumanIL6E. coliNative≥ 95 %Active
50136-MNAEMouse E. coliNative> 90 %Active
62006-WNAESus scrofa (Pig) E. coliNative> 95 %Active
80076-RNAERat E. coliNative> 85 %Active
90197-CNAERhesus E. coliNative> 85 %Active
11821-HNAEHumanIL7E. coliNative> 95 % (HPLC verified)Active
50217-MNAEMouse E. coliNative> 90 %Active
80329-RNAERat E. coliNative> 90 %Active
CT011-H08HHumanIL12HEK293 CellsC-His> 95 %Active
CT011-H08H-BHuman HEK293 CellsC-His> 95 % 
CT021-M02HMouse HEK293 CellsC-hFc> 85 %Active
CT022-M08HMouse HEK293 CellsC-His> 90 %Active
CT023-M0802HMouse HEK293 CellsC-His & C-hFc> 85 %Active
CT024-M0208HMouse HEK293 CellsC-His & C-hFc> 85 %Active
CT045-C08HRhesus HEK293 CellsC-His> 95 % 
CT050-H08HHuman HEK293 CellsC-His> 95 %Active
CT050-HNAHHuman HEK293 CellsNative> 95 %Active
10360-H07EHumanIL15E. coliN-His> 95 %Active
10360-HNCEHuman E. coliN-cleavage> 95 %Active
10584-HNAEHumanIL21E. coliNative> 95 % (HPLC verified)Active
50137-MNAEMouse E. coliNative> 97 %Active
70016-DNAECanine E. coliNative> 97 %Active
80200-R08BRat Baculovirus-Insect CellsC-His> 90 %Active
80200-RNAERat E. coliNative> 95 % 

 

Other Related Proteins

  • CD127
  • CD27
  • BCMA
  • CD62L
  • TGF-β1
  • CD28
  • CD19